Isolation and characterization of the actinomycete, Nocardiopsis, from Haizhou Bay, and its application as a probiotic for Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

Abstract Actinomycetes, as the main producers of a variety active metabolites and enzymes, have received extensive attention in aquaculture. An actinomycete had been isolated from Haizhou Bay identified by morphological analysis 16S rRNA sequencing. Results showed that highest similarity (99.65%) with Nocardiopsis sp. was named strain E10. Strain E10 produced an exo-enzyme proteinase effect strong antibacterial activity against Vibrio anguillarum . This tested probiotic for culture Exopalaemon carinicauda After having fed diet supplemented 30 days, final body weight (Wt), gain rate (WGR), special growth (SGR), survival (SR) digestive enzymes (chymotrypsin, α -amylase lipase) E. all experimental groups no significant differences ( ). However, antioxidant (total superoxide dismutase, catalase peroxidase) non-specific immune-related (acid phosphatase, alkaline phosphatase lysozyme) treatment increased significantly ) compared control group. The cumulative mortality (35.56% 5 × 10 9 CFU/100 g) lower than (73.33%) post challenge V. 14 days. results indicated on growth, but improvement immunity study provides data ecological aquaculture